1DUH
CRYSTAL STRUCTURE OF THE CONSERVED DOMAIN IV OF E. COLI 4.5S RNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0302, 1.3366, 1.3369, 0.9968, 1.3359 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.697, 69.697, 84.102 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.500 - 2.700 |
| R-factor | 0.244 |
| Rwork | 0.230 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.500 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.076 | 0.593 |
| Total number of observations | 154595 * | |
| Number of reflections | 12371 | |
| <I/σ(I)> | 14.5 | 2.1 |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 303.15 | CRYSTALLIZED FROM 1.60-1.90 M (NH4)2SO4, 0.09 M MAGNESIUM ACETATE, 0.05 M SODIUM CACODYLATE PH 6.0, AT 303 K. CRYSTALS WERE STABILISED AT 292 K IN A SOLUTION OF 2.20 M (NH4)2SO4, 0.01 M MGCL2, 0.05 M BIS-TRIS-HCL PH 6.0. SOAK CONDITIONS: CRYSTALS WERE SOAKED IN STABILISATION SOLUTION CONTAINING 0.002 M LUTETIUM CHLORIDE HEXAHYDRATE. CRYOPROTECTION CONDITIONS: AFTER ADDITION OF 20% GLYCEROL (W/V) TO THE SOAK SOLUTION, CRYSTALS WERE FLASH-FROZEN IN LIQUID NITROGEN., VAPOR DIFFUSION, SITTING DROP, temperature 303.15K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MAGNESIUM ACETATE | ||
| 2 | 1 | 1 | SODIUM CACODYLATE | ||
| 3 | 1 | 1 | BIS-TRIS-HCL | ||
| 4 | 1 | 1 | (NH4)2SO4 | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 1 | LUCL3 | ||
| 7 | 1 | 2 | (NH4)2SO4 | ||
| 8 | 1 | 2 | BIS-TRIS-HCL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.6-1.8 (M) | |
| 2 | 1 | reservoir | 90 (mM) | ||
| 3 | 1 | reservoir | Na cacodylate | 50 (mM) | |
| 4 | 1 | reservoir | 2 (mM) |
