1DUD

DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDE HYDROLASE (D-UTPASE) COMPLEXED WITH THE SUBSTRATE ANALOGUE DEOXYURIDINE 5'-DIPHOSPHATE (D-UDP)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	H 3
Unit cell lengths	86.640, 86.640, 62.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.2
Rwork	0.200
RMSD bond length	0.017
RMSD bond angle	27.900 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	4.480 *
High resolution limit [Å]	2.250	2.250 *
Rmerge	0.072	0.304 *
Total number of observations	33369 *
Number of reflections	7520
Completeness [%]	67.0	35 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	4.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	3.4 (mg/ml)
2	1	1	succinate	7 (mM)
3	1	1	PEG8000	1.5 (%)