1DUC

EIAV DUTPASE DUDP/STRONTIUM COMPLEX

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-10
Detector	MARRESEARCH
Spacegroup name	P 41 3 2
Unit cell lengths	106.700, 106.700, 106.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.050
R-factor	0.1686 *
Rwork	0.167
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dup
RMSD bond length	0.019
RMSD bond angle	0.040
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.124	0.686
Total number of observations	142838 *
Number of reflections	13598
<I/σ(I)>	17.7	3.8
Completeness [%]	100.0	100
Redundancy	10.5	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7	4 *	DROP: 3.0 MG/ML PROTEIN, 0.05 M IMIDAZOL MALATE BUFFER, PH 7.0, 21% PEG 400, 20 MM SRCL2, 5 MM DUDP; WELL: 0.1 M IMIDAZOLE MALATE BUFFER, PH 7.0, 42% PEG 400

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.5-1.6 (mg/ml)
2	1	drop		20 (mM)
3	1	drop	dUDP	5 (mM)
4	1	reservoir	PEG400	21 (%(v/v))