1DU0
ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 128 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01-24 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.900, 45.550, 71.620 |
| Unit cell angles | 90.00, 119.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.26900 * |
| RMSD bond length | 0.007 |
| RMSD bond angle | 18.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.039 | 0.351 |
| Total number of observations | 115772 * | |
| Number of reflections | 23204 | |
| Completeness [%] | 95.5 | 70.3 |
| Redundancy | 4.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | bis-Tris-propane, PEG 400, ammonium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium acetate | ||
| 2 | 1 | 1 | bis-Tris-propane | ||
| 3 | 1 | 1 | PEG 400 | ||
| 4 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | duplex DNA | dissolved in 3.5 micro litter of 3M ammonium acetate | |
| 4 | 1 | reservoir | PEG400 | 1 (%) | |
| 5 | 1 | reservoir | ammonium acetate | 0.25 (M) |
