1DTY

CRYSTAL STRUCTURE OF ADENOSYLMETHIONINE-8-AMINO-7-OXONANOATE AMINOTRANSFERASE WITH PYRIDOXAL PHOSPHATE COFACTOR.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-01-01
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	58.280, 56.270, 121.930
Unit cell angles	90.00, 96.96, 90.00

Refinement procedure

Resolution	10.000 - 2.140
R-factor	0.196
Rwork	0.196
R-free	0.32800
RMSD bond length	0.010
RMSD bond angle	0.017
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.520	2.210
High resolution limit [Å]	2.140	2.140
Rmerge	0.092	0.420
Number of reflections	43620
<I/σ(I)>	12.4
Completeness [%]	100.0	96.2
Redundancy	4.1	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	MethoxyPEG 5000 in Bis-tris propane/HC buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	MethoxyPEG 5000 in Bis-tris propane/HC buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K