1DTT
CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH PETT-2 (PETT130A94)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 288 |
Detector technology | IMAGE PLATE |
Collection date | 1996-01-01 |
Detector | FUJI |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 140.700, 110.800, 73.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.000 |
R-factor | 0.199 * |
Rwork | 0.199 |
R-free | 0.27600 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.095 | 0.592 |
Total number of observations | 86903 * | |
Number of reflections | 21409 | 1771 * |
<I/σ(I)> | 11.4 | |
Completeness [%] | 89.2 | 81.7 * |
Redundancy | 4.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 4 * | Stammers, D.K., (1994) J.Mol.Biol., 242, 586. * |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 4 * | Stammers, D.K., (1994) J.Mol.Biol., 242, 586. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 26 (mg/ml) | |
2 | 1 | drop | PEG3400 | 6 (%(w/v)) | |
3 | 1 | reservoir | PEG3400 | 6 (%(w/v)) | |
4 | 1 | drop | citrate/phosphate |