1DTS

CRYSTAL STRUCTURE OF AN ATP DEPENDENT CARBOXYLASE, DETHIOBIOTIN SYNTHASE, AT 1.65 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	73.200, 49.200, 61.800
Unit cell angles	90.00, 107.10, 90.00

Refinement procedure

Resolution	5.500 - 1.650
R-factor	0.169
Rwork	0.169
R-free	0.23000
RMSD bond length	0.015
RMSD bond angle	2.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.650 *
Rmerge	0.076 *
Total number of observations	34422 *
Number of reflections	21608 *
Completeness [%]	84.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	20 (mg/ml)
2	1	reservoir		0.2 (M)
3	1	reservoir	PEG8000	13-18 (%)
4	1	reservoir	cacodylate	0.1 (M)