1DTJ
CRYSTAL STRUCTURE OF NOVA-2 KH3 K-HOMOLOGY RNA-BINDING DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.500, 61.100, 97.300 |
| Unit cell angles | 90.00, 98.20, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.229 |
| Rwork | 0.225 |
| R-free | 0.27900 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.088 | 0.197 |
| Number of reflections | 25590 | |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 99.0 | 99.2 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 3 | 4 * | (NH4)2SO4, MPD, sodium chloride, pH 3.0, VAPOR DIFFUSION, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 2 | 1 | reservoir | MPD | 2 (%) | |
| 3 | 1 | reservoir | Formic acid | 50 (mM) |
