1DT9
THE CRYSTAL STRUCTURE OF HUMAN EUKARYOTIC RELEASE FACTOR ERF1-MECHANISM OF STOP CODON RECOGNITION AND PEPTIDYL-TRNA HYDROLYSIS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Detector technology | CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.080, 77.080, 194.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
Rwork | 0.246 |
R-free | 0.31400 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.000 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.050 | 0.406 |
Total number of observations | 82505 * | |
Number of reflections | 16712 * | |
<I/σ(I)> | 8.3 | |
Completeness [%] | 99.2 | 98 |
Redundancy | 4.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | protein was mixed with equal volume of reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | HEPES | 100 (mM) | |
3 | 1 | reservoir | PEG4000 | 14-22 (%(w/v)) | |
4 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
5 | 1 | reservoir | 200 (mM) |