1DT4
CRYSTAL STRUCTURE OF NOVA-1 KH3 K-HOMOLOGY RNA-BINDING DOMAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1998-03-01 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 44.670, 44.670, 84.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.245 * |
Rwork | 0.222 |
R-free | 0.31000 * |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.800 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.041 | 0.190 |
Number of reflections | 2473 | |
<I/σ(I)> | 24.3 | |
Completeness [%] | 91.2 | 90.3 |
Redundancy | 4.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 20 * | Li2SO4, sodium acetate, sodium cloride, pH 4.0, VAPOR DIFFUSION, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 1.8 (M) | ||
2 | 1 | reservoir | 100 (mM) | ||
3 | 1 | drop | protein | 10 (mg/ml) | |
4 | 1 | drop | 300 (mM) | ||
5 | 1 | drop | HEPES | 20 (mM) |