1DSX
KV1.2 T1 DOMAIN, RESIDUES 33-119, T46V MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 1999-08-20 |
Detector | ADSC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.390, 78.398, 126.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.234 * |
Rwork | 0.237 |
R-free | 0.27900 |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.750 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.048 | 0.371 |
Number of reflections | 97159 | |
<I/σ(I)> | 13.8 | |
Completeness [%] | 96.1 * | 84.4 |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.5 | 18 * | 22% PEG 1500, 5 % ISOPROPANOL, 200 MM NA ACETATE, 12 MM SRCL2, 50 MM TRIS, PH 8.5, pH 8.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-10 (mg/ml) | |
2 | 1 | reservoir | PEG1500 | 22 (%) | |
3 | 1 | reservoir | isopropanol | 5 (%) | |
4 | 1 | reservoir | 200 (mM) | ||
5 | 1 | reservoir | 12 (mM) | ||
6 | 1 | reservoir | Tris-HCl | 50 (mM) |