1DSS
STRUCTURE OF ACTIVE-SITE CARBOXYMETHYLATED D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM PALINURUS VERSICOLOR
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 280 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1990-11 |
| Detector | WEISSENBERG |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.100, 99.610, 80.690 |
| Unit cell angles | 90.00, 114.40, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.880 |
| R-factor | 0.175 * |
| Rwork | 0.172 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HOLO-GAPDH FROM PALINURUS VERSICOLOR (2.0A) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.920 * |
| Data reduction software | WEIS |
| Data scaling software | WEIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.079 | 0.079 |
| Number of reflections | 78030 | |
| <I/σ(I)> | 4.2 | 2.1 |
| Completeness [%] | 94.0 | 56.5 |
| Redundancy | 1.07 | 1.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.1 | 17 * | Song, S.Y., (1983) J.Mol.Biol., 171, 225. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | NAD+ | 0.5 (mM) | |
| 2 | 1 | drop | EDTA | 1.0 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 1.6 (M) | |
| 4 | 1 | drop | phosphate | 0.1 (M) | |
| 5 | 1 | drop | enzyme | 0.035 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 2.7 (M) | |
| 7 | 1 | reservoir | phosphate | 0.1 (M) |
