1DSN

D60S N-TERMINAL LOBE HUMAN LACTOFERRIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Detector technology	IMAGE PLATE
Detector	RIGAKU IIC
Spacegroup name	C 1 2 1
Unit cell lengths	110.200, 57.000, 55.200
Unit cell angles	90.00, 97.60, 90.00

Refinement procedure

Resolution	20.000 - 2.050
R-factor	0.175
RMSD bond length	0.014
RMSD bond angle	1.200
Data reduction software	RIGAKU
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.050 *	2.050 *
Rmerge	0.052
Total number of observations	49951 *
Number of reflections	17803 *
Completeness [%]	83.6 *	70.6 *
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	60 (mg/ml)
2	1	2	Tris-HCl	0.01 (M)
3	1	2	isopropanol	12 (%(v/v))