1DS7
A MINOR FMN-DEPENDENT NITROREDUCTASE FROM ESCHERICHIA COLI B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 1993-03-01 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.740, 57.740, 275.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 * - 2.060 |
R-factor | 0.208 |
Rwork | 0.203 |
R-free | 0.26700 |
RMSD bond length | 0.007 |
RMSD bond angle | 24.900 * |
Data scaling software | PROFILE |
Phasing software | X-PLOR |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.850 * |
Rmerge | 0.096 * |
Total number of observations | 127222 * |
Number of reflections | 24109 * |
Completeness [%] | 81.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | Peg 8000, potassium phosphate, NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 20 (%) | |
2 | 1 | reservoir | potassium phosphate | 50 (mM) |