1DRH
ISOMORPHOUS CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE, 5-DEAZAFOLATE AND 5,10-DIDEAZATETRAHYDROFOLATE: MECHANISTIC IMPLICATIONS
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.943, 61.943, 107.524 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.208 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.000 |
| Refinement software | TNT |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | L-histidine hydrochloride | 0.5 (M) | |
| 2 | 1 | drop | DHFR-ligand complex | 0.050ml | |
| 3 | 1 | drop | calcium acetate | 0.5 (M) | |
| 4 | 1 | reservoir | Tris | 0.1 (M) | |
| 5 | 1 | reservoir | ethanol | 15-25 (%) |
