1DR9
CRYSTAL STRUCTURE OF A SOLUBLE FORM OF B7-1 (CD80)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-24 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 57.280, 57.280, 298.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.550 - 3.000 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.28000 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.114 | 0.480 |
| Total number of observations | 105456 * | |
| Number of reflections | 5204 | |
| <I/σ(I)> | 24 | |
| Completeness [%] | 100.0 * | 99 * |
| Redundancy | 19.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 * | 293 | PEG400, Na Hepes, CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | |
| 2 | 1 | drop | 140 (mM) | ||
| 3 | 1 | drop | 0.05 (%) | ||
| 4 | 1 | reservoir | PEG400 | 28 (%) | 10%-90% v/v dilution |
| 5 | 1 | reservoir | sodium HEPES | 0.1 (M) | 10%-90% v/v dilution |
| 6 | 1 | reservoir | 0.2 (M) | 10%-90% v/v dilution |
