1DR8
STRUCTURE OF MODIFIED 3-ISOPROPYLMALATE DEHYDROGENASE AT THE C-TERMINUS, HD177
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1999-10-21 |
| Detector | WEISSENBERG |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.890, 85.980, 54.370 |
| Unit cell angles | 90.00, 100.30, 90.00 |
Refinement procedure
| Resolution | 6.500 - 2.700 |
| R-factor | 0.201 * |
| Rwork | 0.205 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ipmdh a172l (pdb 1osj) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 29.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR ((ONLINE) 98.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.500 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.051 | 0.203 |
| Number of reflections | 14939 | 685 * |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 86.7 | 79 |
| Redundancy | 15198 | 685 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 25 * | PEG400, sodium acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-7.5 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 0.05 (M) | |
| 3 | 1 | drop | PEG400 | 20 (%(v/v)) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG400 | 40 (%(v/v)) |
