1DR2

2.3 ANGSTROMS CRYSTAL STRUCTURE OF CHICKEN LIVER DIHYDROFOLATE REDUCTASE COMPLEXED WITH THIONADP+ AND BIOPTERIN

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	89.020, 48.240, 64.110
Unit cell angles	90.00, 124.63, 90.00

Refinement procedure

Resolution	? - 2.300
R-factor	0.158
RMSD bond length	0.016
RMSD bond angle	0.800
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.030 *
Total number of observations	24985 *
Number of reflections	8627 *
Completeness [%]	87.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	cDHFR	15 (mg/ml)
2	1	drop	thioNADP+	2 (mM)
3	1	drop	calcium acetate	10 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	drop	Tris-HCl	20 (mM)
6	1	reservoir	ethanol	23 (%(v/v))
7	1	reservoir	calcium acetate	10 (mM)
8	1	reservoir	Tris-HCl	20 (mM)