1DQR

CRYSTAL STRUCTURE OF RABBIT PHOSPHOGLUCOSE ISOMERASE, A GLYCOLYTIC ENZYME THAT MOONLIGHTS AS NEUROLEUKIN, AUTOCRINE MOTILITY FACTOR, AND DIFFERENTIATION MEDIATOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1996-07-01
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	82.695, 115.272, 271.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.500
R-factor	0.211
Rwork	0.212
R-free	0.26500
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.600
High resolution limit [Å]	2.500	2.510
Rmerge	0.041	0.128
Number of reflections	41256
<I/σ(I)>	25.4
Completeness [%]	90.9	90
Redundancy	7.1	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	25 *	13% PEG 8000, 250 mM magnesium acetate, 100 mM sodium cacodylate, 5 mM 6-phosphogluconate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	13 (%)
2	1	reservoir	magnesium acetate	250 (mM)
3	1	reservoir	sodium cacodylate	100 (mM)