1DQR
CRYSTAL STRUCTURE OF RABBIT PHOSPHOGLUCOSE ISOMERASE, A GLYCOLYTIC ENZYME THAT MOONLIGHTS AS NEUROLEUKIN, AUTOCRINE MOTILITY FACTOR, AND DIFFERENTIATION MEDIATOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 1996-07-01 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.695, 115.272, 271.841 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.500 |
R-factor | 0.211 |
Rwork | 0.212 |
R-free | 0.26500 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.510 |
Rmerge | 0.041 | 0.128 |
Number of reflections | 41256 | |
<I/σ(I)> | 25.4 | |
Completeness [%] | 90.9 | 90 |
Redundancy | 7.1 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 25 * | 13% PEG 8000, 250 mM magnesium acetate, 100 mM sodium cacodylate, 5 mM 6-phosphogluconate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 13 (%) | |
2 | 1 | reservoir | magnesium acetate | 250 (mM) | |
3 | 1 | reservoir | sodium cacodylate | 100 (mM) |