1DQQ

CRYSTAL STRUCTURE OF ANTI-LYSOZYME ANTIBODY HYHEL-63

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-06-06
Detector	MACSCIENCE
Spacegroup name	P 1
Unit cell lengths	39.940, 67.340, 84.390
Unit cell angles	81.52, 77.04, 87.60

Refinement procedure

Resolution	100.000 - 1.800
R-factor	0.21
Rwork	0.210
R-free	0.25500
RMSD bond length	0.005
RMSD bond angle	27.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.049	0.315 *
Total number of observations	145275 *
Number of reflections	68027 *
<I/σ(I)>	16
Completeness [%]	86.5	54.5
Redundancy	2.1	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	16 (%(w/v))
3	1	reservoir		0.1 (M)
4	1	reservoir	Tris-HCl	0.1 (M)