1DQL

CRYSTAL STRUCTURE OF AN UNLIGANDED (NATIVE) FV FROM A HUMAN IGM ANTI-PEPTIDE ANTIBODY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	288
Detector technology	AREA DETECTOR
Collection date	1990-11-19
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	53.570, 61.130, 42.200
Unit cell angles	90.00, 96.90, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.191
Rwork	0.181
R-free	0.26100
RMSD bond length	0.007
RMSD bond angle	27.700 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.750
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.145	0.167
Number of reflections	7111
<I/σ(I)>	12.4
Completeness [%]	83.4 *	40.6 *
Redundancy	2.25	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7	295	6-8% (W/V) PEG 8000, 0.05% (W/V) SODIUM AZIDE, pH 7.0, MICROSEEDING, SITTING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	45-50 (mg/ml)
2	1	1	sodium azide	0.05 (%(w/v))
3	1	1	PEG8000	6-8 (%(w/v))