1DQF
CRYSTAL STRUCTURE OF HELIX II OF THE X. LAEVIS SOMATIC 5S RRNA WITH A CYTOSINE BULGE IN TWO CONFORMATIONS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 263 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 57.150, 57.150, 43.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.260 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.570 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.077 |
Number of reflections | 3757 |
<I/σ(I)> | 14 |
Completeness [%] | 94.8 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | MGCL2, CACL2, CACODYLATE, MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MGCL2 | ||
2 | 1 | 1 | CACL2 | ||
3 | 1 | 1 | CACODYLATE | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.2 (mM) | |
2 | 1 | drop | 0.6 (mM) | ||
3 | 1 | drop | MPD | 4.4 (%(v/v)) | |
4 | 1 | drop | sodium cacodylate | 20.2 (mM) | |
5 | 1 | drop | 4.4 (mM) | ||
6 | 1 | reservoir | MPD | 50 (%) |