1DQ3
CRYSTAL STRUCTURE OF AN ARCHAEAL INTEIN-ENCODED HOMING ENDONUCLEASE PI-PFUI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-25 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.550, 85.280, 65.410 |
| Unit cell angles | 90.00, 112.94, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.191 * |
| Rwork | 0.192 |
| R-free | 0.23200 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.054 | 0.255 |
| Number of reflections | 31152 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 93.7 * | 84.9 |
| Redundancy | 2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.5 | 20 * | PEG4000, bis tris, sodium chloride, dioxane, glycerol, pH 6.5, MICRODIALYSIS, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | Bis-Tris-HCl | 50 (mM) | |
| 3 | 1 | 1 | 0.5 (M) | ||
| 4 | 1 | 1 | PEG4000 | 5 (%(w/v)) | |
| 5 | 1 | 1 | dioxane | 10 (%(v/v)) | |
| 6 | 1 | 1 | glycerol | 10 (%) | |
| 7 | 1 | 1 | 2-mercaptoethanol | 2 (mM) |
