1DPZ
STRUCTURE OF MODIFIED 3-ISOPROPYLMALATE DEHYDROGENASE AT THE C-TERMINUS, HD711
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-05-24 |
| Detector | WEISSENBERG |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.430, 87.570, 70.890 |
| Unit cell angles | 90.00, 100.50, 90.00 |
Refinement procedure
| Resolution | 6.800 - 2.800 |
| R-factor | 0.17 * |
| Rwork | 0.177 |
| R-free | 0.26000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IPMDH A172L (PDB 1OSJ) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 29.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (98.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.100 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.071 | 0.340 |
| Number of reflections | 19524 | 616 * |
| <I/σ(I)> | 15.3 | |
| Completeness [%] | 57.6 | 57.6 * |
| Redundancy | 616 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 25 * | PEG400, SODIUM ACETATE, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 0.05 (M) | |
| 2 | 1 | drop | PEG400 | 10 (%(v/v)) | |
| 3 | 1 | drop | protein | 5-7.5 (mg/ml) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG400 | 20 (%(v/v)) |
