1DPY

THREE-DIMENSIONAL STRUCTURE OF A NOVEL PHOSPHOLIPASE A2 FROM INDIAN COMMON KRAIT AT 2.45 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-09-14
Detector	MARRESEARCH
Spacegroup name	R 3 2
Unit cell lengths	57.980, 57.980, 57.980
Unit cell angles	92.02, 92.02, 92.02

Refinement procedure

Resolution	20.000 * - 2.400 *
R-factor	0.202
Rwork	0.202
R-free	0.27100 *
RMSD bond length	0.007
RMSD bond angle	22.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.600
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.092 *	0.174 *
Number of reflections	4629
<I/σ(I)>	7.2
Completeness [%]	93.0 *	92 *
Redundancy	17.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	8 *	NACL, TRIS HCL, EDTA, NAN3, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 281K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		1.4 (M)
4	1	drop		1 (mM)
5	1	reservoir	Tris-HCl	50 (mM)
6	1	reservoir		3.5 (M)