1DPX

STRUCTURE OF HEN EGG-WHITE LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	77.050, 77.050, 37.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.650
R-factor	0.1874 *
Rwork	0.187
R-free	0.24650 *
RMSD bond length	0.010 *
RMSD bond angle	2.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.038	0.143
Total number of observations	176694 *
Number of reflections	13962
<I/σ(I)>	51
Completeness [%]	99.7	100
Redundancy	12.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	293	SODIUM ACETATE, SODIUM CHLORIDE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	Tris	50 (mM)
3	1	reservoir	MPD	70 (%(v/v))