1DPT
D-DOPACHROME TAUTOMERASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1996-11 |
Detector | RIGAKU |
Spacegroup name | P 3 |
Unit cell lengths | 84.230, 84.230, 40.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.540 |
R-factor | 0.164 |
Rwork | 0.164 |
R-free | 0.19200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HUMAN MACROPHAGE MIGRATION INHIBITORY FACTOR |
RMSD bond length | 0.008 |
RMSD bond angle | 27.300 * |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.620 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.036 | 0.276 |
Total number of observations | 104959 * | |
Number of reflections | 43724 | |
<I/σ(I)> | 15.4 | 2.4 |
Completeness [%] | 90.9 | 81.9 |
Redundancy | 2.4 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.8 * | 18 * | pH 5.7 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 100 (mM) | |
3 | 1 | reservoir | PEG4000 | 23 (%(w/v)) | |
4 | 1 | reservoir | sodium citrate | 0.1 (M) | |
5 | 1 | reservoir | ammonium acetate | 0.2 (M) |