1DPR

STRUCTURES OF THE APO-AND METAL ION ACTIVATED FORMS OF THE DIPHTHERIA TOX REPRESSOR FROM CORYNEBACTERIUM DIPHTHERIAE

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	64.000, 64.000, 220.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 3.000
R-factor	0.233 *
Rwork	0.241
R-free	0.41300 *
RMSD bond length	0.024 *
RMSD bond angle	4.800 *
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	3.000 *
Rmerge	0.075 *
Total number of observations	21855 *
Number of reflections	9355
Completeness [%]	83.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		Schiering, N., (1994) J.Mol.Biol., 244, 654. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	ammonium sulfate	1.6 (M)
3	1	drop	PEG200	2 (%)
4	1	drop	dithiothreitol	1 (mM)
5	1	drop	HEPES	100 (mM)
6	1	reservoir	ammonium sulfate	1.6 (M)
7	1	reservoir	PEG200	2 (%)
8	1	reservoir	DTT	1 (mM)
9	1	reservoir	HEPES	100 (mM)