1DPP
DIPEPTIDE BINDING PROTEIN COMPLEX WITH GLYCYL-L-LEUCINE
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Detector technology | IMAGE PLATE |
Collection date | 1993-02-17 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 61 |
Unit cell lengths | 182.570, 182.570, 211.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.200 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.24000 |
RMSD bond length | 0.008 |
RMSD bond angle | 25.000 * |
Data reduction software | DENZO |
Phasing software | X-PLOR (3.0) |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | |
High resolution limit [Å] | 3.200 | 3.200 * |
Rmerge | 0.105 | |
Total number of observations | 170663 * | |
Number of reflections | 57483 | |
Completeness [%] | 88.0 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.2 | pH 6.2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | depeptide | 1 (mM) | |
3 | 1 | reservoir | sodium citrate | 1.49 (M) | |
4 | 1 | reservoir | ethanol | 1 (%) |