1DPP
DIPEPTIDE BINDING PROTEIN COMPLEX WITH GLYCYL-L-LEUCINE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-02-17 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 61 |
| Unit cell lengths | 182.570, 182.570, 211.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.200 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.24000 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.000 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 3.200 | 3.200 * |
| Rmerge | 0.105 | |
| Total number of observations | 170663 * | |
| Number of reflections | 57483 | |
| Completeness [%] | 88.0 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 | pH 6.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | depeptide | 1 (mM) | |
| 3 | 1 | reservoir | sodium citrate | 1.49 (M) | |
| 4 | 1 | reservoir | ethanol | 1 (%) |
