1DPN
B-DODECAMER CGCGAA(TAF)TCGCG WITH INCORPORATED 2'-DEOXY-2'-FLUORO-ARABINO-FURANOSYL THYMINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1999-02-15 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.623, 39.732, 65.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.950 |
| R-factor | 0.16 |
| Rwork | 0.171 |
| R-free | 0.15900 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.005 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.000 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.047 | |
| Total number of observations | 291875 * | |
| Number of reflections | 39151 | |
| Completeness [%] | 94.3 | 70.2 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 293 | SODIUM CACODYLATE, MAGNESIUM ACETATE, SPERMINE, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | MAGNESIUM ACETATE | ||
| 3 | 1 | 1 | SPERMINE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | decamer | 1.25 (mM) | single strand |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | drop | spermine | 6 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | 40 (mM) | ||
| 6 | 1 | reservoir | MPD | 35 (%(v/v)) |
