1DPF
CRYSTAL STRUCTURE OF A MG-FREE FORM OF RHOA COMPLEXED WITH GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-C |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 91.200, 91.200, 56.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.800 - 2.000 |
| Rwork | 0.201 |
| R-free | 0.25200 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.750 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 1.970 |
| High resolution limit [Å] | 2.000 * | 1.900 |
| Rmerge | 0.072 * | 0.227 * |
| Total number of observations | 176768 * | |
| Number of reflections | 16338 * | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 97.8 * | 93.7 * |
| Redundancy | 6.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 4 * | PEG400, citrate, Lithium sulfate, magnesium chloride,, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 10 | 1 | reservoir | 1.2 (M) | ||
| 11 | 1 | reservoir | PEG400 | 10 (%) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | 2-mercaptoethanol | 2.5 (mM) | |
| 5 | 1 | drop | 0.5 (mM) | ||
| 6 | 1 | drop | citrate | 50 (mM) | |
| 7 | 1 | drop | 0.6 (M) | ||
| 8 | 1 | drop | PEG400 | 5 (%) | |
| 9 | 1 | reservoir | citrate | 100 (mM) |
