1DPC

CRYSTALLOGRAPHIC AND ENZYMATIC INVESTIGATIONS ON THE ROLE OF SER558, HIS610 AND ASN614 IN THE CATALYTIC MECHANISM OF AZOTOBACTER VINELANDII DIHYDROLIPOAMIDE ACETYLTRANSFERASE (E2P)

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1992-11-17
Detector	SIEMENS-NICOLET X100
Spacegroup name	F 4 3 2
Unit cell lengths	224.877, 224.877, 224.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.184
Rwork	0.184
RMSD bond length	0.012
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.600 *
Rmerge	0.072 *
Total number of observations	36952 *
Number of reflections	13594 *
Completeness [%]	93.3 *
Redundancy	3.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	50 (mM)
3	1	drop	azide	0.02 (%)
4	1	drop	ammonium sulfate	12-16 (%)
5	1	reservoir	ammonium sulfate	12-16 (%)
6	1	reservoir	HEPES	50 (mM)