1DP4

DIMERIZED HORMONE BINDING DOMAIN OF THE ATRIAL NATRIURETIC PEPTIDE RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-16
Detector	CUSTOM-MADE
Spacegroup name	P 41 21 2
Unit cell lengths	120.250, 120.250, 160.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
Rwork	0.198
R-free	0.22500
RMSD bond length	0.010
RMSD bond angle	23.400 *
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	SOLVE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.130
High resolution limit [Å]	2.000	2.000
Rmerge	0.066	0.285
Number of reflections	79776
<I/σ(I)>	32
Completeness [%]	98.3	96.8
Redundancy	11	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	277	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir		0.2 (M)
5	1	reservoir	PEG4000	26 (%)