1DP2
CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RHODANESE AND LIPOATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 300 |
Detector technology | AREA DETECTOR |
Collection date | 1998-06-11 |
Detector | SIEMENS HI-STAR |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.360, 49.310, 41.680 |
Unit cell angles | 90.00, 99.60, 90.00 |
Refinement procedure
Resolution | 55.000 - 2.010 |
R-factor | 0.17 |
Rwork | 0.170 |
R-free | 0.23000 |
RMSD bond length | 0.010 * |
RMSD bond angle | 2.300 |
Data scaling software | SAINT |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 2.200 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.043 | 0.134 |
Total number of observations | 42252 * | |
Number of reflections | 18241 * | |
<I/σ(I)> | 14 | |
Completeness [%] | 85.6 | 68 |
Redundancy | 2.3 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 * | 293 | Gliubich, F., (1996), J. Biol. Chem., 271, 21054. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.8-1.9 (M) | |
2 | 1 | reservoir | sodium phosphate | 30 (mM) |