1DOV
CRYSTAL STRUCTURE OF THE ALPHA-CATENIN DIMERIZATION DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-25 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 136.460, 136.460, 118.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26300 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 14.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | DM |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.082 | 0.322 |
| Total number of observations | 33971 * | |
| Number of reflections | 8574 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 295 | PEG 400, Tris, urea, dithiothreitol, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-40 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 200 (mM) | |
| 3 | 1 | reservoir | urea | 900 (mM) | |
| 4 | 1 | reservoir | PEG400 | 27 (%) | |
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) |
