1DOS

STRUCTURE OF FRUCTOSE-BISPHOSPHATE ALDOLASE

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1992-02-07
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1 21 1
Unit cell lengths	90.530, 73.380, 57.800
Unit cell angles	90.00, 106.60, 90.00

Refinement procedure

Resolution	10.000 - 1.670
R-factor	0.171
Rwork	0.171
R-free	0.20400
RMSD bond length	0.007
RMSD bond angle	21.860 *
Data reduction software	SDMS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.600 *
Rmerge	0.052
Total number of observations	242370 *
Number of reflections	75160 *
Completeness [%]	74.3 *
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	free interface diffusion *	5.5	20 *	pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1		0.2 (M)
3	1	1	HEPES	0.1 (M)
4	1	2	PEG4000	25-26 (%)
5	1	2		0.2 (M)
6	1	2	HEPES	0.1 (M)