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1DOK

MONOCYTE CHEMOATTRACTANT PROTEIN 1, P-FORM

Experimental procedure
Source typeROTATING ANODE
Source detailsRIGAKU RUH2R
Temperature [K]298
Detector technologyIMAGE PLATE
Collection date1995-11-05
DetectorRIGAKU
Spacegroup nameP 43 21 2
Unit cell lengths50.740, 50.740, 107.650
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution10.000 - 1.850
R-factor0.191
Rwork0.191
R-free0.24500
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1dom
RMSD bond length0.014
RMSD bond angle27.700

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareX-PLOR (3.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]10.0001.920
High resolution limit [Å]1.8501.850
Rmerge0.078

*

0.363

*

Number of reflections12318
<I/σ(I)>15.32.15
Completeness [%]97.094.7
Redundancy4.593.82
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

810 MG/ML PROTEIN IN 50 MM TRIS BUFFER PH 7.5-8 EQUILIBRATED AGAINST 50-55% AMMONIUM SULFATE USING HANGING DROP VAPOR DIFFUSION METHOD., pH 8.0, vapor diffusion - hanging drop
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein10 (mg/ml)
21dropTris-HCl50 (mM)
31reservoirammonium sulfate50-55 (%(w/v))

229183

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