1DOI
2FE-2S FERREDOXIN FROM HALOARCULA MARISMORTUI
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 59.180, 59.180, 124.230 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.195 |
Rwork | 0.195 |
Structure solution method | SIR + NATIVE ANOMALOUS SCATTERING SOFTWARE USED : NULL STARTING MODEL FOR MOLECULAR REPLACEMENT: NULL |
RMSD bond length | 0.018 |
RMSD bond angle | 25.900 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.040 * |
Number of reflections | 9760 |
Completeness [%] | 99.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7 | Sussman, J.L., (1979) J.Mol.Biol., 134, 375. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 10 (mg/ml) | |
2 | 1 | 1 | 4 (M) | ||
3 | 1 | 2 | phosphate | 4 (M) |