1DOI

2FE-2S FERREDOXIN FROM HALOARCULA MARISMORTUI

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	P 63 2 2
Unit cell lengths	59.180, 59.180, 124.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.195
Rwork	0.195
Structure solution method	SIR + NATIVE ANOMALOUS SCATTERING SOFTWARE USED : NULL STARTING MODEL FOR MOLECULAR REPLACEMENT: NULL
RMSD bond length	0.018
RMSD bond angle	25.900 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.900
Rmerge	0.040 *
Number of reflections	9760
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7		Sussman, J.L., (1979) J.Mol.Biol., 134, 375. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1		4 (M)
3	1	2	phosphate	4 (M)