1DO1
CARBONMONOXY-MYOGLOBIN MUTANT L29W AT 105K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 105 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-11-03 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.460, 90.460, 45.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 1.500 |
| R-factor | 0.189 * |
| Rwork | 0.189 |
| R-free | 0.21500 |
| Starting model (for MR) | 2mbw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | SAINT ((SIEMENS)) |
| Data scaling software | SAINT ((SIEMENS)) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.043 | 0.139 |
| Total number of observations | 175770 * | |
| Number of reflections | 32053 | |
| <I/σ(I)> | 14.6 | 5.1 |
| Completeness [%] | 94.5 | 82.3 |
| Redundancy | 5.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 * | 292 | CRYSTALS WERE GROWN IN 2.5M AMMONIUM SULFATE SOLUTION, BUFFERED WITH 20MM TRIS/ HCL TO PH 8.5. THE CRYSTALLIZATION SOLUTION WAS REPLACED IN STEPS AGAINST A SOLUTION CONTAINING 2.5M AMMONIUM SULFATE, 20MM TRIS, 300MG/ML TREHALOSE AT PH 8.5., temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | L29W MbCO | 20 (mM) | |
| 2 | 1 | 1 | glycerol | 75 (%) | |
| 3 | 1 | 1 | potassium phosphate | 25 (%(v/v)) | |
| 4 | 1 | 2 | ammonium sulfate | 2.5 (M) | |
| 5 | 1 | 2 | Tris-HCl | ||
| 6 | 1 | 2 | trehalose | 300 (mg/ml) |
