1DO0
ORTHORHOMBIC CRYSTAL FORM OF HEAT SHOCK LOCUS U (HSLU) FROM ESCHERICHIA COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 208.150, 167.700, 108.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 3.000 |
R-factor | 0.294 |
Rwork | 0.294 |
R-free | 0.34200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HSLU FROM HSLU IN HSLVU CRYSTALS |
RMSD bond length | 0.009 |
RMSD bond angle | 21.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.079 | 0.526 |
Total number of observations | 265668 * | |
Number of reflections | 74040 | |
Completeness [%] | 96.9 | 96 |
Redundancy | 3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | drop consists of equal volume of protein and reservoir solutions in Buffer B * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | Buffer B |
3 | 1 | drop | EDTA | 1 (mM) | Buffer B |
4 | 1 | drop | 1 (mM) | Buffer B | |
5 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
6 | 1 | reservoir | glycerol | 15 (%) | |
7 | 1 | reservoir | PEG8000 | 10.5 (%) | |
8 | 1 | reservoir | ammonium sulfate | 500 (mM) |