1DNS
BASE ONLY BINDING OF SPERMINE IN THE DEEP GROOVE OF THE A-DNA OCTAMER D(GTGTACAC)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-6 |
| Temperature [K] | 277 |
| Detector technology | OSCILLATION CAMERA |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.430, 42.430, 24.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.000 |
| R-factor | 0.115 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 * |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 1365 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
