1DNM
CRYSTAL STRUCTURE AND SEQUENCE-DEPENDENT CONFORMATION OF THE A.G MIS-PAIRED OLIGONUCLEOTIDE D(CGCAAGCTGGCG)
Experimental procedure
Source type | SEALED TUBE |
Temperature [K] | 280 |
Detector technology | DIFFRACTOMETER |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 25.290, 41.780, 64.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 2.500 |
R-factor | 0.193 |
Refinement software | NUCLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.500 |
Rmerge | 0.119 |
Total number of observations | 2495 * |
Number of reflections | 1710 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | other * | 7.2 | 5 * | pH 7.20, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | SPERMINE | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | dodecamer | 0.75 (mM) | |
2 | 1 | drop | 25 (mM) | ||
3 | 1 | drop | spermine | 2.5 (mM) | |
4 | 1 | drop | sodium cacodylate | 30 (mM) | |
5 | 1 | reservoir | MPD | 32 (%(v/w)) |