1DNK

THE X-RAY STRUCTURE OF THE DNASE I-D(GGTATACC)2 COMPLEX AT 2.3 ANGSTROMS RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Detector technology	AREA DETECTOR
Spacegroup name	P 21 21 2
Unit cell lengths	51.100, 108.400, 62.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.188
RMSD bond length	0.016 *
RMSD bond angle	0.039 *
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	18.890
High resolution limit [Å]	2.300
Rmerge	0.076
Number of reflections	12681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		20 *	VAPOR DIFFUSION, HANGING DROP, temperature 293.00K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	WATER
2	1	1	EDTA
3	1	2	WATER

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein
2	1	drop	DNA
3	1	drop		0.3 (M)
4	1	drop	EDTA	20 (mM)
5	1	drop	sodium citrate	50-60 (%sat)
6	1	reservoir		0.3 (M)
7	1	reservoir	EDTA	20 (mM)
8	1	reservoir	sodium citrate	50-60 (%sat)