1DNF
EFFECTS OF 5-FLUOROURACIL/GUANINE WOBBLE BASE PAIRS IN Z-DNA. MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGFG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Detector | NICOLET P3 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.360, 31.150, 45.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.500 |
| R-factor | 0.172 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 0.037 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 2770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 * | pH 9.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | BIS-TRIS-PROPANE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 25 (mM) | |
| 3 | 1 | drop | magnesium chloride | 100 (mM) | |
| 4 | 1 | drop | spermine tetrachloride | 5.6 (mM) | |
| 5 | 1 | drop | MPD | 12.5 (%) | |
| 6 | 1 | reservoir | MPD | 50 (%) |
