1DNE
MOLECULAR STRUCTURE OF THE NETROPSIN-D(CGCGATATCGCG) COMPLEX: DNA CONFORMATION IN AN ALTERNATING AT SEGMENT; CONFORMATION 2
Experimental procedure
| Temperature [K] | 283 |
| Detector technology | DIFFRACTOMETER |
| Detector | NICOLET P3 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.480, 41.260, 66.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.201 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 * |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.200 * |
| Number of reflections | 1848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 10 * | pH 6.50, VAPOR DIFFUSION, temperature 283.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA dodecamer | 1 (mM) | single strand |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 3 | 1 | drop | magnesium chloride | 7.0 (mM) | |
| 4 | 1 | drop | netropsin | 2.0 (mM) | |
| 5 | 1 | drop | spermine tetrachloride | 4.5 (mM) | |
| 6 | 1 | drop | MPD | 5 (%) | |
| 7 | 1 | reservoir | 50 (%) |
