1DN6
THE CRYSTAL STRUCTURE OF D(GGATGGGAG). AN ESSENTIAL PART OF THE BINDING SITE FOR TRANSCRIPTION FACTOR IIIA
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Spacegroup name | P 43 |
| Unit cell lengths | 45.290, 45.290, 24.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.33 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.054 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 3.000 * |
| High resolution limit [Å] | 2.700 |
| Total number of observations | 1786 * |
| Number of reflections | 1459 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 * | 293 | VAPOR DIFFUSION, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 6-20 (%) | |
| 2 | 1 | drop | DNA | 0.2 (mM) | |
| 3 | 1 | drop | magnesium acetate | 12 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 12 (mM) | |
| 5 | 1 | drop | spermine HCl | 0.8 (mM) |
