1DN2
FC FRAGMENT OF HUMAN IGG1 IN COMPLEX WITH AN ENGINEERED 13 RESIDUE PEPTIDE DCAWHLGELVWCT-NH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.541, 60.825, 68.174 |
| Unit cell angles | 90.00, 103.87, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.194 * |
| Rwork | 0.194 |
| R-free | 0.25200 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.640 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.083 | 0.344 |
| Total number of observations | 60434 * | |
| Number of reflections | 14847 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 298 | PEG 4000, isopropanol, sodium acetate, , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 100 (mM) | ||
| 2 | 1 | reservoir | PEG4000 | 20 (%) | |
| 3 | 1 | reservoir | isopropanol | 20 (%) | |
| 4 | 1 | drop | protein | 0.100 (mM) | |
| 5 | 1 | drop | peptide | 0.150 (mM) |
