1DMW
CRYSTAL STRUCTURE OF DOUBLE TRUNCATED HUMAN PHENYLALANINE HYDROXYLASE WITH BOUND 7,8-DIHYDRO-L-BIOPTERIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.357, 107.991, 124.591 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.2 * |
| Rwork | 0.200 |
| R-free | 0.24590 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 22.100 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.311 |
| Total number of observations | 102504 * | |
| Number of reflections | 29570 | 2093 * |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 96.5 | 69.2 |
| Redundancy | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | PEG2000, NaCl, PIPES, Ethylene Glycol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ethylene glycol | 10 (%(v/v)) | cryoprotectant |
| 2 | 1 | drop | 7,8-dihydro-L-biopterin | 1 (mM) | |
| 3 | 1 | reservoir | PEG2000 | 20-30 (%(w/v)) | |
| 4 | 1 | reservoir | PIPES | 40-80 (mM) | pH6.8 |
| 5 | 1 | drop | protain | 4.5 (mg/ml) |
