1DMS
STRUCTURE OF DMSO REDUCTASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Collection date | 1994-06-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 80.700, 80.700, 229.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.880 |
R-factor | 0.169 |
Rwork | 0.169 |
R-free | 0.20300 |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.775 * |
Data reduction software | MOSFLM (V. 5.21-5.23) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.880 * |
Rmerge | 0.059 |
Total number of observations | 227189 * |
Number of reflections | 58291 * |
Completeness [%] | 93.3 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 * | 30 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MPD | 30 (%(v/v)) | |
2 | 1 | drop | PEG4000 | 10 (%(w/v)) | |
3 | 1 | reservoir | MES | 0.1 (M) |