1DM1

2.0 A CRYSTAL STRUCTURE OF THE DOUBLE MUTANT H(E7)V, T(E10)R OF MYOGLOBIN FROM APLYSIA LIMACINA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-06-19
Detector	RIGAKU RAXIS II
Spacegroup name	H 3
Unit cell lengths	89.730, 89.730, 92.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.760 - 1.990
Rwork	0.189
R-free	0.21500 *
RMSD bond length	0.010 *
RMSD bond angle	2.130 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	59.760
High resolution limit [Å]	1.990
Rmerge	0.066
<I/σ(I)>	10.4
Completeness [%]	99.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294 *	SODIUM CITRATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	Na citrate	1.4 (M)
3	1	reservoir	HEPES	0.1 (M)